A Novel Aqueous Micellar Two-Phase System Composed of Surfactant and Sorbitol for Purification of Pectinase Enzyme from Psidium guajava and Recycling Phase Components

Table 2

(a) Partition behavior of pectinase in different surfactant/sorbitol systems. (b) Partition of pectinase in different concentrations of Triton X-100/sorbitol systems.

(a)

System	Concentration of surfactant/sorbitol (% w/w)	TLL (%, w/w)	Selectivity	Purification factor
Triton X-100/sorbitol	16/19	53.1	61.11 ± 0.1^a	3.21 ± 1.1^a
	21/23	52.3	78.11 ± 1.1^b	8.11 ± 1.1^b
	25/26	54.2	86.15 ± 0.1^c	10.2 ± 0.3^c
	30/28	68.8	21.01 ± 1.1^d	3.84 ± 0.2^d
Tween-80/sorbitol	18/16	31.2	29.20 ± 1.1^e	4.33 ± 1.1^ab
	21/20	34.3	28.12 ± 0.2^e	3.12 ± 1.1^e
	23/23	41.2	22.11 ± 0.1^ed	2.81 ± 0.1^d
	28/24	44.3	18.11 ± 0.2^e	3.62 ± 0.3^e
SDS/sorbitol	14/12	32.1	3.23 ± 1.1^j	1.12 ± 0.4^g
	19/17	31.4	3.15 ± 1.3^k	0.07 ± 0.2^h
	23/20	44.2	2.11 ± 0.1^k	0.26 ± 0.3^i
	28/24	56.3	1.42 ± 0.1^jk	0.02 ± 1.1^j

(b)

Triton X-100 (%, w/w)	Sorbitol (%, w/w)	Purification factor	Yield (%)
25	19	2.11 ± 0.2^a	65.3 ± 0.5^a
25	21	3.21 ± 1.1^b	66.6 ± 0.2^b
25	23	2.15 ± 0.1^c	59.4 ± 1.1^c
25	26	3.64 ± 0.2^d	58.3 ± 0.3^ab
25	28	3.58 ± 1.1^e	73.3 ± 0.2^d
26	19	9.11 ± 0.1^f	82.6 ± 0.1^e
26	21	8.13 ± 1.1^g	83.4 ± 1.1^f
26	23	11.42 ± 1.1^g	91.2 ± 0.3^g
26	26	8.11 ± 0.2^h	78.3 ± 0.1^g
26	28	7.72 ± 0.1^b	77.6 ± 0.2^e
27	19	4.83 ± 1.1^ab	62.1 ± 1.1^f
27	21	3.68 ± 0.1^i	58.1 ± 0.2^g
27	23	3.31 ± 0.2^i	53.2 ± 0.3^h
27	26	2.12 ± 0.1^i	54.3 ± 0.4^i
27	28	0.09 ± 0.2^i	43.2 ± 1.1^j
28	19	1.95 ± 0.1^j	41.3 ± 0.1^k
28	21	1.31 ± 1.1^i	38.3 ± 0.3^jk
28	23	2.82 ± 0.2^k	32.6 ± 0.2^j
28	26	1.11 ± 0.3^l	28.4 ± 1.1^l
28	28	0.02 ± 2.1^m	24.3 ± 0.3^m

The sample sizes for all experiments were three. ^a–mMean values followed by different letters differ significantly (p < 0.05).