Gene Network Analysis of Glucose Linked Signaling Pathways and Their Role in Human Hepatocellular Carcinoma Cell Growth and Survival in HuH7 and HepG2 Cell Lines

Figure 6

Signaling pathways with differential effect on proliferation in HepG2 cells versus HuH7 cells. Real-time proliferation assays on xCELLigence were performed in either low or high glucose serum-free media in presence of either LKB1/AMPK pathway inhibitor Compound C, mitochondrial apoptosis inhibitor reduced glutathione (GSH), LKB1 activator metformin, or NADPH oxidases inhibitor VAS2870. Dose-dependent responses were analyzed in high glucose-serum free media. Representative RTCA experiments are presented as cell index normalized at time of treatment according to time and slopes (mean values ± SEM, ) in HepG2 (left panels) and HuH7 cell lines (right panel).

(a) HepG2

(b) HuH7