BioMed Research International / 2015 / Article / Tab 3 / Research Article
Gene Network Analysis of Glucose Linked Signaling Pathways and Their Role in Human Hepatocellular Carcinoma Cell Growth and Survival in HuH7 and HepG2 Cell Lines Table 3 Pathways tested on real-time experiments.
Pathway Drug activator (A)/inhibitor (I) Highest dose
Time of HepG2 HuH7 Effect IC50 ( Effect IC50 ( AMPK AICAR (A) 2 mM 24 hr −
3 10−3 − 1.1 10−3 Compound C (I) 40 24 hr No effect − 3.3 10−5 JAK1 Butein (I) 50 24 hr − 2 10−5 − Nd JNK SP600125 (I) 100 24 hr − 1.8 10−5 − 2.2 10−5 LKB1 Oligomycin (A) 2 mM 48 hr − 3 10−7 − 2.5 10−7 Metformin (A) 10 mM 24 hr − 7 10−6 − 8 10−4 Mek1/2 U0126 (I) 250 1 hr − 1.1 10−5 − Nd mTOR Rapamycin (I) 200 nM 48 hr No effect No effect Mitochondrial apoptosis GSH (A) 10 24 hr − 10−6 − 1.3 10−4 NFκ B p65 Wedelolactone (I) 10 24 hr − 0.8 10−5 − 10−5 NOX VAS2780 (I) 50 1 hr − 5 10−5 − 1.8 10−5 P38MAPK SD169 (I) 300 24 hr + 9.4 10−5 No effect P42/44 A6355 (I) 90 1 hr − − − − PKA KT5720 (I) 20 48 hr + 1.1 10−4 + Nd PKC P3115 (I) 50 1 hr 5 10−6 − − Nd Pi3K LY294002 (I) 50 48 hr − 3.3 10−5 − 3 10−5 PPARA GW6471 (I) 1 mM 1 hr − 5.7 10−4 − 4 10−4
AMPK, 5′-AMP-activated protein kinase; AICAR: 5-aminoimidazole-4-carboxamide ribonucléotide; GSH: reduced glutathione; JAK1: Janus kinase 1; JNK: Jun-NH2 kinase; LKB1: serine/threonine kinase 11; NFκ Bp65: nuclear factor kappa B p65 subunit; NOX: NADPH oxidases; P38MAPK: P38 mitogen-activated kinase; Pi3K: Pi3 kinase; PKA: protein kinase AMPc-dependant; PKC, protein kinase C; and PPARA, peroxisome-proliferator activated receptor alpha.
