Gene Network Analysis of Glucose Linked Signaling Pathways and Their Role in Human Hepatocellular Carcinoma Cell Growth and Survival in HuH7 and HepG2 Cell Lines

Table 4

Regulation of gene transcription by glucose in HepG2 cells measured by RT-qPCR. Levels of gene expression in high (4.5 g/L) versus low (1 g/L) glucose concentrations.

Symbol	Gene name	Gene ID	Fold change
Upregulated
APOC3	Apolipoprotein C-III	345	2.83 ± 0.08
IRF1	Interferon regulatory factor 1	3659	1.92 ± 0.05
IKBKAP	Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase complex-associated protein	8518	1.73 ± 0.03
Down-regulated
FOXO1A	Forkhead box O1	2308	−9.09 ± 0.02
EGR1	Early growth response 1	1958	−8.33 ± 0.06
CREBBP	CREB binding protein (Rubinstein-Taybi syndrome)	1387	−3.23 ± 0.01
MYC	v-myc myelocytomatosis viral oncogene homolog (avian)	4609	−3.23 ± 0.07
ADIPOR2	Adiponectin receptor 2	79602	−3.03 ± 0.20
BHLHB2	Basic helix-loop-helix domain containing, class B, 2	8552	−2.86 ± 0.06
CDKN1B	Cyclin-dependent kinase inhibitor 1B (p27, Kip1)	6043	−2.27 ± 0.01
SOD2	Superoxide dismutase 2, mitochondrial	6648	−2.04 ± 0.20
MAP4K4	Mitogen-activated protein kinase kinase kinase kinase 4	9448	−1.82 ± 0.06
CEBPB	CCAAT/enhancer binding protein (C/EBP), beta	1051	−1.79 ± 0.02
FOXA2/HNF3b	Forkhead box A	3170	−1.67 ± 0.09
TGFB1	Transforming growth factor, beta 1	7040	−1.61 ± 0.01
TNFRSF1A	Tumor necrosis factor receptor superfamily, member 1A	7132	−1.49 ± 0.01
HES1	Hairy and enhancer of split 1, (drosophila)	3280	−1.45 ± 0.00
HNF4A	Hepatocyte nuclear factor 4, alpha	3172	−1.43 ± 0.05
Not regulated
ADIPOR1	Adiponectin receptor 1	51094
AFP	Alpha fetoprotein	174
CAT	Catalase	847
CEBPA	CCAAT/enhancer binding protein (C/EBP), alpha	1050
FABP1	Fatty acid binding protein 1, liver	2168
FOXM1/HNF3	Forkhead box M1	2305
HK2	Hexokinase 2	3099
LDLR	Low density lipoprotein receptor	3949
PPARG	Peroxisome proliferator-activated receptor gamma	5468
PPARGC1A	Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha	10891
TBP	TATA box binding protein	6908

See Figure 8.