Multi-Leu PACE4 Inhibitor Retention within Cells Is PACE4 Dependent and a Prerequisite for Antiproliferative Activity

Figure 3

⁶⁴Cu/NOTA-ML uptake and retention kinetics in cells. Percentage of the initially applied radioactivity in prostate cancer (a), hepatocellular (b), or fibrosarcoma (c) cell lines after different pulse times. Cells were lysate following two extensive PBS washes. For retention, a 2-hour pulse was performed followed by washes and different efflux time points. Control cells are shown as plain line and knockdown cells as dashed lines. Data are means ± SEM of at least 3 independent experiments.

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