Encapsulation of Curcumin in Diblock Copolymer Micelles for Cancer Therapy

Table 1

Acute toxicity effects of PNPC on hematological and blood chemical indices in mice.

Parameters	Groups
	Control	PNPC; 31.25 mg/kg	PNPC; 62.5 mg/kg	PNPC; 125 mg/kg
Animal weight (g)	24.3 ± 2.2	25 ± 3.1	24.7 ± 2.5	23.2 ± 2.2
RBC (Millin/mm^3)	6.08 ± 0.2	6.8 ± 0.8	7.2 ± 0.5	8.2 ± 0.4^*
HCT (%)	38.4 ± 1.7	37.7 ± 1.2	38.5 ± 2.5	42.5 ± 2^*
Hgb (g/dL)	10 ± 1.2	11.3 ± 1.8	11.7 ± 1.3	13.4 ± 1.6^*
MCV (FL)	51 ± 0.7	50.1 ± 0.8	49.3 ± 1.5	49.1 ± 1.2
MCH (pg)	16.5 ± 0.2	16.4 ± 0.3	15.5 ± 0.4	15.3 ± 0.3
MCHC (mol/L)	32.3 ± 0.7	32.4 ± 0.5	32.2 ± 0.4	32.5 ± 0.3
Plt (1000/mm^3)	567 ± 50	546 ± 91	570 ± 45	540 ± 38
WBC (1000/mm^3)	6.7 ± 0.8	8.7 ± 2	7.9 ± 1.5	8.1 ± 2.2
Neutrophils (%)	35.5 ± 2	36.6 ± 12	38 ± 5	38.2 ± 6
Monocytes (%)	2.1 ± 3	1.2 ± 0.9	3.1 ± 1	2.1 ± 0.8
Lymphocytes (%)	53.5 ± 6.4	52 ± 9	57.2 ± 5	57 ± 4
Na (mM/L)	150.3 ± 2.9	146.5 ± 3.3	154.2 ± 2.1	157.1 ± 1.5^*
K (mM/L)	5.2 ± 0.5	5.8 ± 1.3	6.1 ± 1.7	8.2 ± 2.2^*
Cl (mM/L)	111 ± 4	117 ± 3	114 ± 2	117 ± 3^*
HCO^3− (mM/L)	22.6 ± 3.2	17.3 ± 1.6	17.5 ± 2.2	16.3 ± 2.7^*
Osm (mOsm/kg)	307 ± 4.7	307 ± 5.2	312.2 ± 7	320 ± 8^*
Ca (mM/L)	0.72 ± 0.14	0.63 ± 0.1	0.65 ± 0.2	0.66 ± 0.3
Mg (mM/L)	0.29 ± 0.04	0.30 ± 0.03	0.25 ± 0.04	0.31 ± 0.03
Glu (mg/dL)	194 ± 33	204 ± 48	182 ± 30	176 ± 25
Lac (mM/L)	5.4 ± 1.2	5.2 ± 1.3	5.2 ± 2	5.7 ± 1.8
Urea (mg/dL)	21.2 ± 2.6	21.3 ± 3.6	26 ± 5	43 ± 7^*
Cr (mg/dL)	0.48 ± 0.08	0.51 ± 0.07	0.55 ± 0.07	0.92 ± 0.12^*
AST (U/L)	455 ± 45	538 ± 85	777 ± 80	1280 ± 152^*
ALT (U/L)	68 ± 8.8	70.6 ± 8.7	86 ± 21	258 ± 86^*
ALP (U/L)	763 ± 50	794 ± 67	825 ± 36	1187 ± 92^*
GGT (U/L)	5.5 ± 0.5	—	—	6.2 ± 0.6
ALB (mg/dL)	3 ± 0.2	—	—	2.8 ± 0.4
T.BIL (mg/dL)	0.5 ± 0.03	—	—	0.47 ± 0.05
D.BIL (mg/dL)	0.35 ± 0.05	—	—	0.32 ± 0.04

Values are means ± SEM. < 0.05 compared to control. PNPC = the polymeric nanoparticle curcumin, RBC = red blood cell, HCT = hematocrit, MCV = mean corpuscular volume, MCH = mean corpuscular hemoglobin, MCHC = mean corpuscular hemoglobin concentration, WBC = white blood cells, Plt = platelets, Na = sodium, K = potassium, Cl = chloride, HCO³⁻ = bicarbonate, Osm = osmolarity, Ca = calcium, Mg = magnesium, Cr = creatinine, Lac = lactate, Glu = glucose, AST = aspartate transaminase, ALT = alanine transaminase, ALP = alkaline phosphatase, GGT = gamma-glutamyl transpeptidase, ALB = albumin, T.BIL = total bilirubin, and D.BIL = direct bilirubin.