Encapsulation of Curcumin in Diblock Copolymer Micelles for Cancer Therapy

Table 2

Chronic toxicity effects of PNPC on hematological and blood chemical indices and the organs weight percentage in mice.

Parameters	Groups
	Control	PNPC; 15.63 mg/kg	PNPC; 31.25 mg/kg	PNPC; 62.5 mg/kg
Animal weight (g)	24.3 ± 2.2	24 ± 3.2	23.3 ± 3.4	18.3 ± 2.7^*
RBC (Millin/mm^3)	6.08 ± 0.08	6.3 ± 0.07	6.9 ± 0.3	7.7 ± 0.4^*
HCT (%)	38.4 ± 1.7	36.6 ± 2.1	37.4 ± 1.2	40.3 ± 3.2
Hgb (g/dL)	10 ± 1.2	12 ± 1.1	9.8 ± 0.9	12.3 ± 1
MCV (FL)	51 ± 0.7	52.8 ± 0.6	50.4 ± 2	49.5 ± 1.5
MCH (pg)	16.5 ± 0.2	17.3 ± 0.3	15.7 ± 0.9	15.6 ± 0.5
MCHC (mol/L)	32.3 ± 0.7	32.8 ± 0.4	32 ± 0.7	32.2 ± 0.6
Plt (1000/mm^3)	567 ± 50	519 ± 75	539 ± 53	594 ± 41
WBC (1000/mm^3)	6.7 ± 0.8	6.9 ± 1	6.5 ± 1.8	7.7 ± 2.1
Neutrophils (%)	32.5 ± 2	41 ± 8	38.5 ± 11	42 ± 9
Monocytes (%)	2.1 ± 3	4.4 ± 2.1	1.8 ± 1	3.8 ± 2.2
Lymphocytes (%)	66.5 ± 6.4	53 ± 11	60.8 ± 5.8	51 ± 13
Na (mM/L)	150.3 ± 2.9	149.7 ± 3.2	151.2 ± 3.8	157.4 ± 1.5^*
K (mM/L)	5.2 ± 0.5	5.7 ± 1.6	4.9 ± 0.4	4.3 ± 0.8
Cl (mM/L)	111 ± 4	112.2 ± 2.1	112.7 ± 1.8	115.5 ± 2.3
HCO^3− (mM/L)	22.6 ± 3.2	16.7 ± 4.9	19.9 ± 3.2	18.9 ± 2.7
Osm (mOsm/kg)	307 ± 4.7	301.3 ± 12	306.9 ± 8.2	315.8 ± 7
Ca (mM/L)	0.72 ± 0.14	0.61 ± 0.14	0.74 ± 0.07	0.97 ± 0.6
Mg (mM/L)	0.29 ± 0.04	0.30 ± 0.02	0.32 ± 0.04	0.35 ± 0.03
Glu (mg/dL)	194 ± 33	174 ± 31	172 ± 40	212 ± 30
Lac (mM/L)	5.4 ± 1.2	4.8 ± 1	5.1 ± 1.1	4.3 ± 1.5
Urea (mg/dL)	21.2 ± 2.6	19.4 ± 3.2	20 ± 2.6	24.5 ± 3.8
Cr (mg/dL)	0.48 ± 0.08	0.42 ± 0.05	0.53 ± 0.03	0.98 ± 0.08^*
AST (U/L)	455 ± 45	423 ± 64	503 ± 33	585 ± 36^*
ALT (U/L)	68 ± 8.8	72.3 ± 5.9	76.3 ± 12	92.3 ± 5^*
ALP (U/L)	763 ± 50	633 ± 83	666 ± 85	207 ± 23^*
GGT (U/L)	5.5 ± 0.5	—	5.9 ± 0.8	6.4 ± 1
ALB (mg/dL)	3 ± 0.2	—	3.4 ± 0.6	2.3 ± 0.3^*
T.BIL (mg/dL)	0.5 ± 0.03	—	0.47 ± 0.13	0.56 ± 0.1
D.BIL (mg/dL)	0.35 ± 0.05	—	0.38 ± 0.08	0.34 ± 0.05
% body weight
Heart	0.52 ± 0.06			0.47 ± 0.04
Liver	6.3 ± 0.35			4.2 ± 0.26^*
Spleen	0.43 ± 0.05			0.58 ± 0.07
Lung	0.73 ± 0.09			0.71 ± 0.07
Kidney	1.53 ± 0.15			1.46 ± 0.22
Brain	1.12 ± 0.11			1.19 ± 0.15

Values are means ± SEM. < 0.05 compared to control. PNPC = the polymeric nanoparticle curcumin, RBC = red blood cell, HCT = hematocrit, MCV = mean corpuscular volume, MCH = mean corpuscular hemoglobin, MCHC = mean corpuscular hemoglobin concentration, WBC = white blood cells, Plt = platelets, Na = sodium, K = potassium, Cl = chloride, HCO³⁻ = bicarbonate, Osm = osmolarity, Ca = calcium, Mg = magnesium, Cr = creatinine, Lac = lactate, Glu = glucose, AST = aspartate transaminase, ALT = alanine transaminase, ALP = alkaline phosphatase, GGT = gamma-glutamyl transpeptidase, ALB = albumin, T.BIL = total bilirubin, and D.BIL = direct bilirubin.