The Sea Lamprey as an Etiological Model for Biliary Atresia

Figure 7

Taurine reduced the degeneration of extrahepatic bile duct. 3D reconstructed confocal images of liver during developmental biliary atresia in sea lamprey. Cysteine sulfinic acid decarboxylase (CSAD) siRNA treatment facilitated degeneration of the extrahepatic bile duct (white arrows). Blue immunofluorescence staining: caspase 3 (apoptosis marker). Green immunofluorescence staining: BCL2 (apoptosis protection marker). Red immunofluorescence staining: CSAD (the rate limiting enzyme for taurine biosynthesis). Whole livers were processed with a modified CLARITY method [122]. Scale bar: 500 μm.

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