Delivery of Nucleic Acids and Nanomaterials by Cell-Penetrating Peptides: Opportunities and Challenges

Table 2

Examples of cellular uptake markers and organelle markers for green fluorescent CdSe/ZnS quantum dots in live cell imaging.

Marker	Function	Color (Ex/Em)	Incubation time	Conc.
FM4-64	Endocytosis marker	Red (506/750)	15 min	2 M
Alexa Fluor 568-Transferrin	Clathrin-dependent endocytosis marker	Red (580/630)	5 min	25 g/mL
Alexa Fluor 568-Cholera toxin B	Caveolae-dependent endocytosis marker	Red (580/630)	10 min	5 g/mL
Texas red-Neural Dextran 70	Macropinocytosis marker	Red (595/615)	30 min	5 M
TMR-Dextran	Early endosome marker	Red (555/580)	5 min	10 mg/mL
Lyso Tracker Red DND 99	Lysosome marker	Red (577/590)	10 min	0.5 M
EEA1-Alexa Fluor 568	Early endosome marker	Red (580/630)	5 min	10 g/mL
Lamp-1-Alexa Fluor 568	Late endosome marker	Red (578/603)	5 min	10 g/mL
Mito Tracker Deep 633	Mitochondria marker	Red (640/662)	10 min	1 M
Hoechst 33342	Nuclei marker	Blue (352/461)	30 min	5 M