Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers

Figure 1

UPLC chromatograms of the monitored ion transitions for 2′-deoxycytidine (dC), 5-methyl-2′-deoxycytidine (5mdC), and 5-hydroxymethyl-2′-deoxycytidine (5hmdC): (a) blood sample from a volunteer, (b) saliva sample from the same volunteer, and (c) calibration standard, containing dC (30.7 ng/mL), 5mdC (1.5 ng/mL), and 5hmdC (0.07 ng/mL).

(a)

(b)

(c)