Fabrication of an Amperometric Flow-Injection Microfluidic Biosensor Based on Laccase for In Situ Determination of Phenolic Compounds

Figure 3

Effect of temperature (a), pH (b), and injection flow rate (c) on the current response of the microfluidic biosensor. Error bars describe the standard deviation of the three replicates. Solution: acetate buffer 0.1 M (pH 5.0.) as the running solution and ABTS 25 μM in acetate buffer 0.1 M (pH 5.0.) as the analyte.

(a)

(b)

(c)