Fabrication of an Amperometric Flow-Injection Microfluidic Biosensor Based on Laccase for In Situ Determination of Phenolic Compounds

<div>Current-time response curve with background signal subtracted (a) and calibration curve (b) of the microfluidic biosensor. Increasing concentrations of ABTS (from 0.5 <i>μ</i>m to 200 <i>μ</i>M) in 0.1 M acetate buffer (pH 4.5) were injected at 200 <i>μ</i>L min<sup>−1</sup>. Potential applied of 0.4 V at room temperature. Error bars describe the standard deviation of the three replicates.</div>

BioMed Research International

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Figure 4

Figure 4: Fabrication of an Amperometric Flow-Injection Microfluidic Biosensor Based on Laccase for In Situ Determination of Phenolic Compounds 