Research Article
Phylogenetic Groups of Escherichia coli Strains from Patients with Urinary Tract Infection in Iran Based on the New Clermont Phylotyping Method
Table 1
Primer sequences used in the extended quadruplex phylotyping method [
6].
| | PCR reaction | Primer ID | Target | Primer sequence | PCR product (bp) |
| | Quadruplex | chuA.1b | chuA | 5-ATGGTACCGGACGAACCAAC-3 | 288 | | chuA.2 | 5-TGCCGCCAGTACCAAAGACA-3 | | yjaA.1b | yjaA | 5-CAAACGTGAAGTGTCAGGAG-3 | 211 | | yjaA.2b | 5-AATGCGTTCCTCAACCTGTG-3 | | TspE4C2.1b | TspE4C2 | 5-CACTATTCGTAAGGTCATCC-3 | 152 | | TspE4C2.2b | 5-AGTTTATCGCTGCGGGTCGC-3 | | AceK.f | arpA | 5-AACGCTATTCGCCAGCTTGC-3 | 400 | | ArpA1.r | 5-TCTCCCCATACCGTACGCTA-3 |
| | Group E | ArpAgpE.f | arpA | 5-GATTCCATCTTGTCAAAATATGCC-3 | 301 | | ArpAgpE.r | 5-GAAAAGAAAAAGAATTCCCAAGAG-3 |
| | Group C | trpAgpC.1 | trpA | 5-AGTTTTATGCCCAGTGCGAG-3 | 219 | | trpAgpC.2 | 5-TCTGCGCCGGTCACGCCC-3 |
| | Internal control | trpBA.f | trpA | 5-CGGCGATAAAGACATCTTCAC-3 | 489 | | trpBA.r | 5-GCAACGCGGCCTGGCGGAAG-3 |
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