MicroRNA-381 Negatively Regulates TLR4 Signaling in A549 Cells in Response to LPS Stimulation

Figure 4

miR-381 prompts NF-κB activation and increases inflammatory gene expression, partially through targeting IκBα. (a) The overexpression of miR-381 significantly increased the luciferase activity of NF-κB. Transfection of A549 cells with miR-381 mimics markedly enhanced the level of (b) COX-2, (c) TNFα, and (d) IL-6. The inhibition of miR-381 obviously reduced the level of (e) COX-2, (f) TNFα, and (g) IL-6. (h) The upregulation of COX-2, IL-6, and TNFα induced by LPS treatment could be partially reversed through the inhibition of miR-381 expression. (i) The miR-381 inhibitor failed to suppress NF-κB activation in A549 cells transfected with si-IκBα. independent experiments. , .

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