Research Article

Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors

Figure 2

Purification of integrase enzyme. (a) Purified protein fractions were analyzed by SDS-PAGE and stained with Coomassie Blue. Lane 1: molecular weight markers; 2: crude extracts; 3: fraction of HIV-1 IN eluted from the His Trap column revealed minor contaminating species. (b) Purified IN was performed by the Western blotting by using anti-His mAb and anti-HIV-1 IN mAb as primary antibody.