Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors

<div>Purification of integrase enzyme. (a) Purified protein fractions were analyzed by SDS-PAGE and stained with Coomassie Blue. Lane 1: molecular weight markers; 2: crude extracts; 3: fraction of HIV-1 IN eluted from the His Trap column revealed minor contaminating species. (b) Purified IN was performed by the Western blotting by using anti-His mAb and anti-HIV-1 IN mAb as primary antibody.</div>

BioMed Research International

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Figure 2

Figure 2: Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors 