The Protective Effect of Melatonin on Neural Stem Cell against LPS-Induced Inflammation

Figure 3

The measurement of neurosphere size in LPS-induced inflammation. (a) Neurosphere was observed using bright field microscopy and neurosphere size was measured using Image J software. Neurosphere sizes were maintained in the melatonin treatment group compared to the control group. In LPS-induced inflammation, melatonin maintained neurosphere size compared to the LPS (100 ng/mL) only treatment group. (b) The graph indicated the percentages of neurosphere size in all groups. The percentage of neurospheres greater than 300 μm was higher in the melatonin treatment group compared to the normal control group. In LPS-induced inflammation, the percentage of neurospheres greater than 200 μm was higher in the melatonin group compared with the LPS only treatment group. Scale bar: 200 μm. (c) The number of neurospheres whose diameter was over 60 μM was counted by Image J software program. The number of neurospheres was reduced under LPS-induced inflammation and was increased by melatonin treatment in spite of inflammatory condition. Data were expressed as mean ± S.E.M, and each experiment included 3 repeats per condition. Differences were considered significant at (compared to the control group).

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