Figure 2: Seminative SDS-PAGE of crude extracts of the bacterial soil isolates (1–8), followed by zymography using glycol-chitin (a), chitosan DA 56% (b), or chitosan DA35% (c) as a substrate. A known chitinolytic strain of Bacillus licheniformis (B.L) was used as a positive control. The positions of marker proteins (M) are given on the sides of the gels.