Endogenous Two-Photon Excited Fluorescence Provides Label-Free Visualization of the Inflammatory Response in the Rodent Spinal Cord

Figure 2

Comparison of endogenous TPEF after SCI and microglial markers at a cellular level. Magnification of the lesion border 21 d after SCI. (a) Multiphoton image of an unlabeled cryosection obtained by combining SHG (blue channel), TPEF (green channel), and CARS (red channel). (b) CARS channel shown in gray scale; the dotted line indicates the border between the strongly damaged tissue area and the more preserved contralateral white matter. (c) The TPEF channel shown after thresholding in black and white. (d) Immunohistochemical staining of the same tissue section as shown in (a–c). Overlay of DAPI staining (blue channel), CD68 (green channel), and Iba1 (red channel). (e) Iba1 immunoreactivity shown in black and white. (f) CD68 immunoreactivity shown in black and white. Scale bar: 100 μm.

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