Shear Stress Induces Differentiation of Endothelial Lineage Cells to Protect Neonatal Brain from Hypoxic-Ischemic Injury through NRP1 and VEGFR2 Signaling

Figure 3

The immunofluorescence staining revealed the improvement of neurovascular structure with ELCs treatments in HI injured brain. The number of neurons was increased in cortex area with both ASCs and ELCs treatments using NeuN staining (a). The significant preservation of vascular structure was observed in both cortex and hippocampus by RECA staining in ELCs transplantation (b). The staining of hChromatin (hChrom) indicated the homing of transplanted cells into the damaged brain (c). The triple staining of hChrom (green) with vessels (RECA, red) and all cell nuclei (DAPI, blue) indicated the engraftment of transplanted ELCs to the vascular structure. The angiogenesis was assessed by isolectin B4 (IB4) staining and showed promising enhancement in ELCs treatments (d). The colocalization of IB4 (red) with RECA (green) indicated the promotion of angiogenesis after application of ELCs. , Scale bar: 100 μm.

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