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BioMed Research International
Volume 2015 (2015), Article ID 864169, 8 pages
http://dx.doi.org/10.1155/2015/864169
Research Article

Proteomic Analysis of Human Brain Microvascular Endothelial Cells Reveals Differential Protein Expression in Response to Enterovirus 71 Infection

1Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China
2Department of Clinical Laboratory, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China
3Central Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou 510623, China
4Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
5Clinical Laboratory, The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China
6Saban Research Institute, Children’s Hospital Los Angeles, University of Southern California, Los Angeles, CA 90027, USA

Received 13 November 2014; Accepted 16 January 2015

Academic Editor: Amogh A. Sahasrabuddhe

Copyright © 2015 Wenying Luo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

2D DIGE technology was employed on proteins prepared from human brain microvascular endothelial cells (HBMEC), to study the differentially expressed proteins in cells at 0 h, 1 h, 16 h, and 24 h after infection. Proteins found to be differentially expressed were identified with matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDITOF/TOF MS) analysis. We identified 43 spots showing changes of at least 2.5 fold up- or downregulated expressions in EV71-infected cells at different time when comparing to control, and 28 proteins could be successfully identified by MALDI TOF/TOF mass spectrometry analysis. 4 proteins were significantly upregulated, and 6 proteins were downregulated, another 18 proteins were different expression at different incubation time. We identified changes in the expression of 12 cellular metabolism-related proteins, 5 molecules involved in cytoskeleton, 3 molecules involved in energy metabolism, 2 molecules involved in signal transduction, 1 molecule involved in the ubiquitin-proteasome pathway, 1 molecule involved in cell cycle, 1 molecule involved in apoptosis-related protein, 1 molecular chaperone, and 2 unknown proteins. These findings build up a comprehensive profile of the HBMEC proteome and provide a useful basis for further analysis of the pathogenic mechanism that underlies EV71 infections to induce severe neural complications.