Proteomic Analysis of Human Brain Microvascular Endothelial Cells Reveals Differential Protein Expression in Response to Enterovirus 71 Infection

Table 1

Differential proteins identified by mass spectrometry (MS) after 2D DIGE of in HBMEC from EV71-infected contrast to mock-infected.

Spot number	Protein Name	Gene name	Accession number	Nominal mass (Mr)/calculated PI	Infection time (hpi)			Sequence coverage (%)
					1	16	24
1	Deoxyuridine 5′-triphosphate nucleotidohydrolase	DUT	gi3041664	26975/9.65	—	0	0	11
2		ACBP1	gi10140853	11786/5.41	↑	↓	↓	34
3	L-lactate dehydrogenase B chain OS	LDHB	gi291575128	36900/5.71	↓	↓	↓	8
4	Ubiquitin-conjugating enzyme E2 N	UBE2N	gi4507793	17184/6.13	↑	↓	↑	19
5	Unnamed protein product	Unnamed gene product	gi194375299	40819/5.78	↑	↑	↑	23
6	Protein DJ-1	DJ-1, PARK7	gi31543380	20050/6.33	↓	↓	↓	20
7	Microtubule-associated protein RP/EB family member 1	MAPRE1	gi6912494	30151/5.02	↓	↓	↑	20
8	Proteasome subunit alpha type-5	PSMB5	gi7106387	26579/4.74	↓	↑	↓	43
9	Heat shock protein 27	HSP27	gi662841	22427/7.83	↓	↓	↑	13
10	14-3-3 protein beta/alpha	YWHAB	gi197692221	28209/4.76	↑	↓	↑	28
11	Nuclear chloride channel	CLIC	gi4588526	27249/5.02	↑	↓	↑	16
12	Serine/threonine-protein phosphatase 6 catalytic subunit isoform b	PPP6B	gi4506029	35806/5.43	↓	↓	↓	28
13	Splicing factor, arginine/serine-rich 2	SCAF1	gi62898065	25487/5.12	↑	↑	↑	15
14	Spermidine synthase	SRM	gi531202	34360/5.21	↓	↓	↓	5
15	Serine/threonine-protein phosphatase PP1-beta catalytic subunit isoform 1	PPP1CB	gi4506005	37961/5.84	↑	↓	↓	25
16	Uroporphyrinogen decarboxylase	UROD	gi1322019	41119/5.77	↑	↓	↑	20
17	Ubiquinol-cytochrome c reductase core I protein	UQCRC1	gi515634	53270/5.94	↑	↑	↑	15
18	Glutathione synthetase	GSS	gi4504169	52523/5.67	↑	↑	↑	24
19	Vimentin	VIM	gi62414289	53676/5.06	↓	↓	↓	7
20	Actin, cytoplasmic 1	ACTB	gi4501885	42052/5.29	↑	↑	↑	29
21	Serum albumin	PRO2044	gi6650826	30084/7.70	↓	↑	↑	5
22	Thymosin beta-10	TMSB10	gi339697	5701/6.45	↓	↓	↓	16
23	Transcription factor BTF3 homolog 4	BTF3L4	gi29789195	17260/5.95	↓	↓	↑	5
24	Superoxide dismutase	SOD1	gi4507149	16154/5.70	↓	↓	↑	59
25	Stathmin isoform a	STMN1	gi5031851	17292/5.76	↑	↑	↓	15
26	Inosine triphosphate pyrophosphatase	ITPA	gi13398328	21827/5.78	↓	↓	↑	18
27	Thioredoxin 1	TXN1	gi9280551	11971/4.69	↑	↓	↑	49
28	Cofilin-1	CFL1	gi5031635	18719/8.22	↓	↑	↑	51