Tanshinone IIA Attenuates Renal Fibrosis after Acute Kidney Injury in a Mouse Model through Inhibition of Fibrocytes Recruitment

Figure 6

Tanshinone IIA inhibits MCP-1 expression in the folic acid-injured kidney at day 3. (a) Representative images and semiquantification (b) of MCP-1 (original magnification ×100) stained by immunohistochemistry in the kidney sections at day 3. (c) Representative immunoblot and quantification (d) of MCP-1 in kidney cortical tissue lysates at day 3. Data are expressed as the mean ± SEM (). ^★ versus group TS; ^★★ versus group FA. Notes: monocyte chemotactic protein 1 (MCP-1); Ctrl: mice treated with vehicle alone; TS: mice treated with Tanshinone IIA alone; FA: folic acid-treated mice followed by vehicle treatment; FA + TS: folic acid-treated mice subjected to Tanshinone IIA injection for 3 consecutive days.

(a)

(b)

(c)

(d)