Activity of BKM120 and BEZ235 against Lymphoma Cells

Figure 12

ROS generation in cells was monitored during treatment with BKM120 and BEZ235 alone and in combination with CQ after 3, 9, 12, and 24 hours. The ROS level was determined by flow cytometry, and quantitative analysis of ROS generation is shown in histograms. Coadministration of the antioxidant N-acetyl cysteine (NAC) blocked the increased ROS generation. Results represent the mean ± SD obtained from three independent experiments.