Peripheral Blood Mononuclear Cell Proteome Changes in Patients with Myelodysplastic Syndrome

Table 2

List of spots that differed significantly when RCMD patients were compared with a control group of healthy volunteers.

Spot	Protein	Uniprot AC	Peptides		Fold
1	Vinculin	P18206	5	0.0017	2.7
2	Tubulin alpha-1C chain; tubulin alpha-1B chain	Q9BQE3; P68363	4	0.00478	2.6
	Annexin A6	P08133	2
3	Alpha-enolase	P06733	8	0.026	−2.5
4	Alpha-enolase	P06733	6	0.0345	−2.4
5	Cytosolic nonspecific dipeptidase	Q96KP4	14	0.00179	−2.3
	Tubulin beta chain	P07437	5
6	Annexin A1	P04083	10	0.00387	−2.4
7	Leukotriene A-4 hydrolase	P09960	12	0.0000401	−2.1
8	T-complex protein 1 subunit theta	P50990	6	0.00287	−1.7
9	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	3	0.0133	2.1
10	Plastin-2	P13796	17	0.000467	−3.3
11	Protein S100-A9	P06702	1	0.0259	−2.0
12	Neutral alpha-glucosidase AB	Q14697	3	0.012	−2.0
13	Leukocyte elastase inhibitor	P30740	8	0.0114	−2.0
14	Alpha-enolase	P06733	4	0.000782	−2.0
	Adenylosuccinate synthetase isozyme 2	P30520	5
	Rab GDP dissociation inhibitor beta	P50395	3
	C-terminal-binding protein 1	Q13363	2
15	Annexin A7	P20073	3	0.00019	−2.0
	Adenosylhomocysteinase	P23526	3
	Proliferation-associated protein 2G4	Q9UQ80	1
16	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	3	0.0247	1.9
	T-complex protein 1 subunit beta	P78371	2
17	Vinculin	P18206	5	0.000267	1.9
18	Triosephosphate isomerase	P60174	5	0.000113	−1.7
19	Eukaryotic initiation factor 4A-I	P60842	6	0.000589	−1.9
	Heterogeneous nuclear ribonucleoprotein F	P52597	4
	Heterogeneous nuclear ribonucleoprotein Q	O60506	4
	T-complex protein 1 subunit theta	P50990	3
20	Plastin-2	P13796	14	0.00156	−1.9
21	Alpha-enolase	P06733	7	0.00129	−1.9
22	Plastin-2	P13796	16	0.0363	−1.9
23	Proteasome subunit alpha type-6	P60900	4	0.000319	−1.8
	UPF0568 protein C14orf166	Q9Y224	4
	Hypoxanthine-guanine phosphoribosyltransferase	P00492	2
24	Alpha-enolase	P06733	6	0.0172	1.8
25	Ubiquitin carboxyl-terminal hydrolase 5	P45974	5	0.00228	−1.7
26	Proteasome activator complex subunit 1	Q06323	2	0.0017	1.7
	Coagulation factor XIII A chain	P00488	2
27	Heat shock cognate 71 kDa protein	P11142	4	0.00034	−1.8
	Rho-related GTP-binding protein RhoC	P08134	2
28	40S ribosomal protein SA	P08865	7	0.00126	−1.8
29	Triosephosphate isomerase	P60174	7	0.0217	−1.8
30	T-complex protein 1 subunit zeta	P40227	4	0.00972	−1.8
31	WD repeat-containing protein 1	O75083	9	0.0183	−1.8
32	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	2	0.00152	1.7
	Heterogeneous nuclear ribonucleoprotein K	P61978	2
	ADP-sugar pyrophosphatase	Q9UKK9	2
33	Coronin-1A	P31146	2	0.00433	−1.7
34	26S proteasome non-ATPase regulatory subunit 14	O00487	2	0.000458	−1.7
35	m7GpppX diphosphatase	Q96C86	4	0.00316	−1.5
	Transaldolase	P37837	3
36	Proliferation-associated protein 2G4	Q9UQ80	3	0.000667	−1.7
	Annexin A1	P04083	2
37	Macrophage-capping protein	P40121	3	0.0279	−1.7
38	Talin-1	Q9Y490	5	0.00497	1.7
39	Heat shock 70 kDa protein 4	P34932	5	0.0282	−1.7
40	Plastin-2	P13796	17	0.0131	−1.6
41	Glutathione S-transferase P	P09211	6	0.000628	−1.8
42	Protein S100-A7	P31151	2	0.00218	−1.6
43	Talin-1	Q9Y490	4	0.00804	1.6
44	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform	P30153	7	0.00245	−1.6
45	Ribonuclease inhibitor	P13489	3	0.0247	−1.6
46	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	3	0.00181	1.6
47	Tubulin beta-1 chain	Q9H4B7	2	0.0144	1.6
48	Vinculin	P18206	3	0.0104	1.6
49	Annexin A5	P08758	12	0.00129	−1.8
50	Proteasome activator complex subunit 2	Q9UL46	3	0.000338	−1.6
51	Annexin A6	P08133	20	0.000508	−2.6
	Heat shock 70 kDa protein 1A/1B	P08107	3
	Heat shock 70 kDa protein 1-like	P34931	2
	Heat shock protein HSP 90-alpha	P07900	2
52	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	2	0.000127	−1.6
	Glutathione S-transferase P	P09211	3
53	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	2	0.0352	1.5
54	Triosephosphate isomerase	P60174	2	0.00181	−1.5
55	Ras GTPase-activating-like protein IQGAP1	P46940	13	0.0219	−1.7
56	Actin, cytoplasmic 1; Actin, cytoplasmic 2	P60709; P63261	2	0.019	1.5
57	Rab GDP dissociation inhibitor beta	P50395	3	0.0336	−1.5
	26S proteasome non-ATPase regulatory subunit 11	O00231	2
58	Rab GDP dissociation inhibitor beta	P50395	13	0.0000838	−1.5
	Alpha-enolase	P06733	6
	Adenylosuccinate synthetase isozyme 2	P30520	5
	Beta-centractin	P42025	3
59	Tubulin alpha-1C chain	Q9BQE3	5	0.0393	1.5
	Tubulin alpha-1A chain	Q71U36	5
	Tubulin alpha-4A chain	P68366	4
60	Alpha-enolase	P06733	7	0.0459	1.5
	Phosphoglycerate kinase 1	P00558	8
61	Lymphocyte-specific protein 1	P33241	2	0.00972	−1.5
	Secernin-1	Q12765	3
62	Eukaryotic translation initiation factor 3 subunit H	O15372	3	0.00335	−1.5
	T-complex protein 1 subunit eta	Q99832	4
63	L-Lactate dehydrogenase B chain	P07195	8	0.00156	−1.5
	Pyruvate kinase isozymes M1/M2	P14618	5
64	6-Phosphogluconolactonase	O95336	5	0.0029	−1.5
	Endoplasmic reticulum resident protein 29	P30040	4
65	Pyruvate kinase isozymes M1/M2	P14618	8	0.000801	−1.7
	Sorting nexin-6	Q9UNH7	2
66	Proteasome subunit beta type-3	P49720	3	0.00454	−1.5
	Triosephosphate isomerase	P60174	5
67	Pyruvate kinase isozymes M1/M2	P14618	16	0.0219	−1.5
68	14-3-3 protein zeta/delta	P63104	6	0.0053	−1.6
69	4-trimethylaminobutyraldehyde dehydrogenase	P49189	2	0.0183	−1.4
	Caspase-1	P29466	2
70	Proteasome subunit beta type-4	P28070	2	0.000337	−1.4
71	Chloride intracellular channel protein 1	O00299	6	0.00012	−1.7
	Tumor protein D5	O43399	2
72	Major vault protein	Q14764	8	0.0122	−1.7
	Ubiquitin-like modifier-activating enzyme 1	P22314	8
73	Transitional endoplasmic reticulum ATPase	P55072	13	0.0132	−1.8
	Ras GTPase-activating-like protein IQGAP1	P46940	3
74	Serum albumin	P02768	10	0.00454	−1.7
75	Heat shock protein beta-1	P04792	2	0.00187	1.5
76	Vinculin	P18206	6	0.00886	−1.5
77	Gelsolin	P06396	7	0.0459	−2.1
78	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	5	0.0221	1.9
	Annexin A1	P04083	3
79	Tryptophan-tRNA ligase, cytoplasmic	P23381	7	0.00185	−1.6
80	Vinculin	P18206	7	0.0369	−1.7
81	Proteasome activator complex subunit 1	Q06323	7	0.0328	−1.5
82	Cofilin-1	P23528	3	0.000586	−1.7
83	Endoplasmin	P14625	5	0.00491	−2.6
84	Alpha-enolase	P06733	4	0.000166	2.4
	Monoglyceride lipase	Q99685	1
85	Alpha-actinin-1	P12814	2	0.00777	2.4
86	Heterogeneous nuclear ribonucleoprotein H2	P55795	4	0.000792	−2.3
	Heterogeneous nuclear ribonucleoprotein H	P31943	3
87	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	3	0.00752	2.3
	Alpha-actinin-1	P12814	2
88	Delta(3,5)-delta(2,4)-dienoyl-CoA isomerase, mitochondrial	Q13011	3	0.000218	−2.2
	Estradiol 17-beta-dehydrogenase 8	Q92506	2
89	Heterogeneous nuclear ribonucleoproteins A2/B1	P22626	3	0.000986	−2.2
90	Transitional endoplasmic reticulum ATPase	P55072	5	0.00163	−2.1
91	Aconitate hydratase, mitochondrial	Q99798	7	0.000362	−2.1
	Endoplasmin	P14625	2
92	Endoplasmin	P14625	7	0.000319	−2.0
93	Heat shock 70 kDa protein 1A/1B	P08107	4	0.00155	−2.0
	Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial	P10515	6
	Stress-70 protein, mitochondrial	P38646	3
	Annexin A6	P08133	3
94	Heat shock protein 75 kDa, mitochondrial	Q12931	3	0.00144	−2.0
95	78 kDa glucose-regulated protein	P11021	17	0.0018	−2.5
	Protein disulfide-isomerase A4	P13667	4
	Endoplasmin	P14625	2
96	Endoplasmic reticulum resident protein 29	P30040	6	0.00318	−1.9
	ATP synthase subunit gamma, mitochondrial	P36542	2
97	Endoplasmin	P14625	7	0.0109	−1.9
98	Tropomyosin alpha-4 chain	P67936	4	0.00755	2.1
99	78 kDa glucose-regulated protein	P11021	7	0.00377	−1.8
100	Endoplasmin	P14625	5	0.0108	−1.8
101	40S ribosomal protein SA	P08865	3	0.00000707	−1.8
	Vimentin	P08670	4
102	F-actin-capping protein subunit beta	P47756	2	0.000949	−1.8
103	78 kDa glucose-regulated protein	P11021	15	0.00946	−2.1
104	Superoxide dismutase [Cu-Zn]	P00441	1	0.00139	1.7
	ATP synthase subunit alpha, mitochondrial	P25705	1
105	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	4	0.00138	1.7
106	F-actin-capping protein subunit alpha-1	P52907	2	0.00247	1.7
107	Purine nucleoside phosphorylase	P00491	2	0.00353	−1.7
	Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 1	P04843	2
108	Stress-70 protein, mitochondrial	P38646	10	0.00224	−1.6
	Heat shock cognate 71 kDa protein	P11142	4
109	Stress-70 protein, mitochondrial	P38646	2	0.0137	1.7
110	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	2	0.00201	1.7
111	Delta(3,5)-delta(2,4)-dienoyl-CoA isomerase, mitochondrial	Q13011	3	0.00338	−1.7
112	60 kDa heat shock protein, mitochondrial	P10809	6	0.00385	1.7
	Integrin beta-3	P05106	4
	ATP synthase subunit beta, mitochondrial	P06576	2
113	Aldehyde dehydrogenase, mitochondrial	P05091	9	0.000949	−1.7
114	Heat shock cognate 71 kDa protein	P11142	8	0.0122	−1.7
	Stress-70 protein, mitochondrial	P38646	4
	Endoplasmin	P14625	4
115	T-complex protein 1 subunit beta	P78371	5	0.0215	−1.7
116	Moesin	P26038	4	0.000902	−1.6
	T-complex protein 1 subunit zeta	P78371	2
117	Macrophage-capping protein	P40121	2	0.0114	−1.6
118	Stress-70 protein, mitochondrial	P38646	13	0.0106	−1.7
	Annexin A6	P08133	3
119	60 kDa heat shock protein, mitochondrial	P10809	11	0.000211	−1.6
	Heterogeneous nuclear ribonucleoprotein K	P61978	2
120	Elongation factor Tu, mitochondrial	P49411	4	0.00392	−1.5
	Isocitrate dehydrogenase [NADP] cytoplasmic	O75874	2
121	Pre-mRNA-processing factor 19	Q9UMS4	2	0.00523	−1.6
122	Prohibitin	P35232	9	0.0121	−1.6
	Annexin A2	P07355	6
123	78 kDa glucose-regulated protein	P11021	6	0.0216	−1.6
	ATP synthase subunit beta, mitochondrial	P06576	4
	Integrin alpha-IIb	P08514	2
124	DnaJ homolog subfamily B member 11	Q9UBS4	2	0.00315	−1.6
	Moesin	P26038	2
125	Protein disulfide-isomerase	P07237	7	0.0422	−1.6
126	Gelsolin	P06396	3	0.0294	1.5
127	14-3-3 protein zeta/delta	P63104	5	0.0337	1.5
	14-3-3 protein eta	Q04917	2
128	60 kDa heat shock protein, mitochondrial	P10809	18	0.00252	−1.5
129	Endoplasmin	P14625	4	0.0251	−1.6
130	Synaptosomal-associated protein 23	O00161	3	0.0254	1.5
131	Stress-induced-phosphoprotein 1	P31948	1	0.0329	−1.5
132	Calreticulin	P27797	2	0.00435	−1.5
133	UPF0568 protein C14orf166	Q9Y224	4	0.0208	−1.5
134	60 kDa heat shock protein, mitochondrial	P10809	7	0.000412	1.5
	ATP synthase subunit beta, mitochondrial	P06576	2
	Integrin beta-3	P05106	2
135	Alpha-enolase	P06733	4	0.0357	−1.5
136	Myosin-9	P35579	5	0.0106	1.5
137	Myosin regulatory light polypeptide 9	P24844	3	0.0325	1.7
	Protein disulfide-isomerase A6	Q15084	2
138	Endoplasmic reticulum resident protein 29	P30040	6	0.0104	−1.5
139	Peroxiredoxin-4	Q13162	2	0.0362	−1.5
140	Tyrosine-protein phosphatase nonreceptor type 6	P29350	3	0.0319	−1.5
	Protein disulfide-isomerase A3	P30101	2
141	Protein disulfide-isomerase A3	P30101	2	0.0125	−1.5
142	PDZ and LIM domain protein 1	O00151	2	0.00306	1.5
143	ATP synthase subunit beta, mitochondrial	P06576	18	0.00266	−1.4
	Protein disulfide-isomerase A6	Q15084	6
	78 kDa glucose-regulated protein	P11021	4
	Protein disulfide-isomerase	P07237	2
	Vimentin	P08670	2
144	Plastin-2	P13796	15	0.000372	−1.6
145	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	3	0.00905	1.5
146	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	4	0.0156	1.8
147	60 kDa heat shock protein, mitochondrial	P10809	9	0.018	1.5
148	Filamin-A	P21333	4	0.000123	2.6
149	Filamin-A	P21333	3	0.00116	2.6
150	Alpha-actinin-1	P12814	7	0.00104	2.4
151	Ficolin-1	O00602	2	0.000967	−2.4
152	Ezrin	P15311	3	0.0136	−2.4
153	Coronin-1A	P31146	1	0.00915	−2.2
154	Alpha-actinin-1	P12814	4	0.00109	2.2
155	Fermitin family homolog 3	Q86UX7	2	0.000261	2.1
156	Filamin-A	P21333	2	0.000568	2.1
157	Alpha-actinin-1	P12814	5	0.0017	2.0
158	Filamin-A	P21333	2	0.0123	1.9
159	Ficolin-1	O00602	2	0.0221	−1.8
160	Alpha-actinin-1	P12814	5	0.000516	1.6
	Filamin-A	P21333	2
161	14-3-3 protein zeta/delta	P63104	3	0.022	1.8
162	Prohibitin	P35232	3	0.0414	−1.8
163	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	2	0.0133	1.7
164	Nucleoprotein TPR	P12270	1	0.00952	1.7
165	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	5	0.037	1.7
	Filamin-A	P21333	3
	Beta-parvin	Q9HBI1	2
166	Alpha-actinin-1	P12814	3	0.00969	1.7
167	Filamin-A	P21333	2	0.0109	1.7
168	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	2	0.00529	2.8
169	Ezrin	P15311	2	0.0174	−1.7
170	Beta-actin-like protein 2	Q562R1	2	0.0226	1.6
171	Filamin-A	P21333	2	0.00975	1.6
172	Filamin-A	P21333	3	0.0319	1.6
173	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	2	0.0202	1.6
174	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	3	0.0207	1.6
175	Myosin-9	P35579	2	0.0225	1.6
176	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	4	0.000991	2.2
177	Filamin-A	P21333	3	0.0131	1.5
178	Filamin-A	P21333	5	0.031	2.1
	Actin, cytoplasmic 1; actin, cytoplasmic 2	P60709; P63261	3