Dissecting the Role of Curcumin in Tumour Growth and Angiogenesis in Mouse Model of Human Breast Cancer

Figure 1

Curcumin inhibits proliferation and enhances apoptosis in MDA.MB231 cancer cells. (a) For wound-healing assay, MDA.MB231 cells were wounded by scratching and monitored over 48 h to determine the rate of wound closure (40x magnification), scale bar, 200 μm. (b) Cell migration was assessed by measuring relative wound closure. Data represent mean ± SEM (; ). At 48 h after wound induction, there were clearly less cells in the denuded area of curcumin treated cells than untreated cells. (c) MTT assay results show a suppression of proliferation in breast cancer cells treated with curcumin respect to control cells S.E. Data represent mean ± SEM (; ). (d) In vitro apoptosis assay by flow cytometry indicated that curcumin (10 μM) enhances apoptosis in MDA.MB231 cells ( value < 0.0001). ((e)-(f)) Western blot showing that curcumin enhances the expression of p53 in MDA.MB231 cells treated with curcumin (lane 2) with respect to controls (lane 1). α-actin was used as loading control.

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