Improving the Understanding of Pathogenesis of Human Papillomavirus 16 via Mapping Protein-Protein Interaction Network

Figure 1

Flowchart to integrate and analyze PPI network between HPV16 and human proteins. A candidate interaction was found, if the human protein had homologs in the human PPI network. This method provided 3,022 candidate interactions. An SVM model was employed to evaluate candidate interactions and 1,121 interactions were left. Subsequently, these interactions were filtered if human proteins with targeted human proteins had the same as cellular component. 1,015 interactions were obtained; positive training set and test set were further filtered by tissue specificity. Finally, 877 interactions were obtained and analyzed. Solid lines delineate validated interactions between virus and human proteins, and dotted lines delineate candidate interactions which would be validated. Homologous proteins are surrounded by ellipse.