Comparative Analysis of Methods to Induce Myocardial Infarction in a Closed-Chest Rabbit Model

Figure 2

Histological analysis of left ventricle lateral wall transmural infarction. Histological views of left ventricle lateral wall infarction focused on left ventricle anterior wall after mid segment circumflex artery alcohol injection (a) and of the interventricular septum focused on the basal segment after proximal segment right coronary artery alcohol injection (b) showing muscle and fibrosis. Heart was excised, cut, then embedded in OCT, flash frozen in liquid nitrogen, and stored at −80°C. Ten-micrometer cryosections were stained to confirm the presence of ischemic tissue.

(a)

(b)