Alleviation of Kainic Acid-Induced Brain Barrier Dysfunction by 4-O-Methylhonokiol in In Vitro and In Vivo Models

Figure 4

Effect of MH, scavengers on KA-induced convulsion in mice. Seizure scores were rated according to the scale devised by Racine. Seizures were induced by KA injection (10–30 mg/kg, i.p.); the mice in the control group received an equal volume of 0.9% NaCl. Male mice were grouped ( or 6/group) and pretreated (i.p. injection) with MH (5–20 mg/kg), scavengers such as trolox (50 mg/kg, i.p.) and DMTU (50 mg/kg, i.p.), or NaCl (0.9%). Fifty minutes after the MH or control pretreatment, seizures in mice in the KA, scavengers + KA, and MH + KA groups were induced by KA injection (10 mg/kg, i.p.); the mice in the control group received an equal volume of 0.9% NaCl. The preconvulsive behavior was scored within 3 h after KA injection. Scores at various time points after seizure induction were analyzed by two independent observers, after which they were averaged and statistically compared by the Holm-Sidak test. All data are presented as means ± SE. , , versus control group. , versus KA group.

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