Alleviation of Kainic Acid-Induced Brain Barrier Dysfunction by 4-O-Methylhonokiol in In Vitro and In Vivo Models

Figure 7

Effect of MH, scavengers on KA-induced neuronal loss and Oxidative stress and [Ca²⁺]_i influx in primary cultured hippocampal cells. Concentration data for KA-induced toxicity in primary cultured hippocampal neurons. Examination of the dose effect of KA on neuronal viability by the MTT assay. Neurons were exposed to KA at concentrations of 30, 50, and 100 μM for 48 h. Cell viability at 48 h after KA exposure was measured by the MTT assay. Examination of the dose effect of KA on neuronal ROS level and [Ca²⁺]_i the DCFH-DA and Ca²⁺ indicator with fura-4 assay. All data are presented as means ± SE. versus control group. , , versus KA group.

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