MUTYH Gene Polymorphisms as Risk Factors for Rheumatoid Arthritis

Table 1

Primers and PCR conditions used in this study to determine MUTYH gene polymorphisms.

Gene	SNP rs number	Position	Alleles	Primers	PCR product size (bp)	PCR conditions; (annealing temperature)	Restriction; enzyme site	DNA; fragment size; (bp)
MUTYH	rs3219463	1:45579019 (promoter region)	A/G	Forward: 5′-TCCCACAAGCCTTTGTAACC-3′; Reverse: 5′-CGTATTGGGAGCTCTGGTGT-3′	347	60°C	SbfI	A: 347; G: 161 + 186
	rs3219476	1:45575257 (intron)	G/T	Forward: 5′-GTTGCAGTGAGCCAAGATCA-3′; Reverse: 5′-AACCTGCCTTTTGTGAGCTG-3′	311	58°C	BtsCI	G: 58 + 172 + 81; T: 58 + 253
	rs3219489	1:45570092 (exon)	G/C	Forward: 5′-TGTGGTGAGCACCAAACCTA-3′; Reverse: 5′-GGCTGTTCCAGAACACAGGT-3′	319	62°C	HpyCH4III	G: 193 + 126; C: 319
	rs3219493	1:45568856 (intron)	G/C	Forward: 5′-CTCAAGTGATCCACCCGACT-3′; Reverse: 5′-AGTGAAGCCTGGAGTGGAGA-3′	332	58°C	HpyCH4V	G: 332; C: 139 + 193