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BioMed Research International
Volume 2015, Article ID 905648, 5 pages
Research Article

Clinical Usefulness of Immunohistochemical Staining of p57kip2 for the Differential Diagnosis of Complete Mole

1Department of Obstetrics and Gynecology, Iino Hospital, 4-3-2 Fuda, Chōfu, Tokyo 182-0024, Japan
2Department of Obstetrics and Gynecology, Showa University Northern Yokohama Hospital and Showa University School of Medicine, 35-1 Chigasaki Chuo, Tsuduki-Ku, Yokohama, Kanagawa 224-8503, Japan
3Department of Pathology, Showa University School of Medicine and Showa University Northern Yokohama Hospital, 35-1 Chigasaki Chuo, Tsuduki-Ku, Yokohama, Kanagawa 224-8503, Japan
4Maternity Clinic Kojima, 4-1-27 Asahi-Cho, Akishima, Tokyo 196-0025, Japan

Received 28 September 2014; Accepted 13 January 2015

Academic Editor: Shalini Rajaram

Copyright © 2015 Shigeru Sasaki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. Can polymer-based immunohistochemical staining of replace DNA analysis as an inexpensive means of differentiating complete mole from partial mole or hydropic abortion? Methods and Materials. Original paraffin-embedded tissue blocks from 14 equivocal cases were turned over to our laboratory and examined by immunohistochemical staining of . Results. Four of the 14 cases showed clearly negative nuclear staining in cytotrophoblasts and villous stromal cells: these results were fully concordant with the control staining. The remaining 10 cases showed apparently positive staining in cytotrophoblasts and villous stromal cells. Without DNA analysis we are able to clearly differentiate the 4 cases of complete mole among the 14 equivocal cases. During follow-up, secondary low-risk gestational trophoblastic neoplasia (GTN) developed in 1 of the 4 cases of complete mole: the GTN was treated by single-agent chemotherapy. No subsequent changes were observed during follow-up in the other cases. Conclusion. Polymer-based immunohistochemical staining of (paternally imprinted gene, expressed from maternal allele) is a very effective method that can be used to differentiate androgenetic complete mole from partial mole and hydropic abortion. We might be able to avoid the cost of DNA analysis.