A Versatile Orthotopic Nude Mouse Model for Study of Esophageal Squamous Cell Carcinoma

Figure 3

Hematoxylin- and eosin-stained (H&E) tissue images. (a) Cross section image of a naïve mouse esophagus. (b) Image of a mouse esophagus inoculated with KYSE150 showing esophageal luminal stricture due to tumor growth indicated by arrow. (c) H&E image of a KYSE150 orthotopic tumor containing multiple tumor nodules (indicated by arrows) with lymphovascular invasion. (d) Image of multiple tumor nodules in human ESCC, similar to what is observed in the mouse orthotopic model shown in (c). (e) Image of poorly differentiated KYSE150 orthotopic tumor indicates invasion from mucosa to adventitia and with lymphovascular permeation in the mouse esophagus at week 4. (f) A magnified view of the tumor invasion edge as indicated in the red-boxed area in (e) showing ulceration in lumen resulting from tumor invasion. (g) A cross-section H&E image of a mouse esophagus inoculated with SLMT-1. Orthotopic tumor is of low histological grade. (h) A magnified view of red-boxed area reveals large tumor nest near the lumen.