Review Article
Biofilm Formation by Helicobacter pylori and Its Involvement for Antibiotic Resistance
Table 1
Generation of CAM resistance mutations in biofilm and planktonic cells. The 2-day and 3-day biofilms and planktonic cells were exposed to the indicated concentrations of CAM (biofilms were exposed to one-eighth, one-quarter, or one-half of the MBC of CAM at concentrations of 0.125, 0.25, and 0.5 μg/mL, concentrations which are equivalent to 8x, 16x, and 32x MIC and planktonic cultures were also exposed to one-quarter or one-half of the MBC of CAM at concentrations of 0.063 and 0.125 μg/mL, concentrations which are equivalent to 4x and 8x MIC) for 24 h under microaerobic conditions at 37°C with shaking. After incubation, cells were recovered in fresh Brucella supplemented with 7% FCS agar, and the generation of CAM resistant mutants was assessed in media supplemented with 1.0 μg/mL CAM. When no CAM resistant cells were detected, exposure to CAM was repeated up to 5 times. The table indicates the accumulation ratio of the generated CAM resistance in biofilms (number of samples was 12 or 13) or in planktonic cultures (number of samples was 12).
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