Research Article
Inhibition of ACE Retards Tau Hyperphosphorylation and Signs of Neuronal Degeneration in Aged Rats Subjected to Chronic Mild Stress
Figure 2
Tau hyperphosphorylation in the hippocampus of stressed rats. (a) Immunohistological detection of the hyperphosphorylated tau protein (anti-PHF) in the hippocampus of a stressed rat (left panel) relative to a nonstressed control (right panel). Nuclei were counterstained with hematoxylin (HE; bar: 200 μm). (b) Immunoblot detection of hyperphosphorylated tau in hippocampal extracts of stressed rats relative to nonstressed controls was performed with anti-PHF antibody (IB: PHF (AT8)). The lower panel is a control immunoblot detecting total tau protein (/group). (c) Immunofluorescence localization of AT1R (green) and PHF (red) in hippocampal CA1 neurons of a stressed rat (bar: 20 μm). The AT1R was detected with affinity-purified rabbit anti-AT1R antibodies followed by F(ab)2 fragments of Alexa Fluor 488-labeled (green) secondary antibodies, and hyperphosphorylated tau was visualized with affinity-purified mouse anti-PHF antibody followed by F(ab)2 fragments of Alexa Fluor 546-labeled secondary antibodies (red). Histological experiments are representative of 4 rats/group ((a) and (c)).
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