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BioMed Research International
Volume 2015 (2015), Article ID 917640, 8 pages
Research Article

Characterization Method for 3D Substructure of Nuclear Cell Based on Orthogonal Phase Images

1Faculty of Science, Jiangsu University, Zhenjiang 212013, China
2School of Mechanical Engineering, Jiangsu University, Zhenjiang 212013, China

Received 12 June 2015; Accepted 6 August 2015

Academic Editor: Kennethhun Mok

Copyright © 2015 Ying Ji et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A set of optical models associated with blood cells are introduced in this paper. All of these models are made up of different parts possessing symmetries. The wrapped phase images as well as the unwrapped ones from two orthogonal directions related to some of these models are obtained by simulation technique. Because the phase mutation occurs on the boundary between nucleus and cytoplasm as well as on the boundary between cytoplasm and environment medium, the equation of inflexion curve is introduced to describe the size, morphology, and substructure of the nuclear cell based on the analysis of the phase features of the model. Furthermore, a mononuclear cell model is discussed as an example to verify this method. The simulation result shows that characterization with inflexion curve based on orthogonal phase images could describe the substructure of the cells availably, which may provide a new way to identify the typical biological cells quickly without scanning.