|
| Study | Specimen | patients | Validation group | Radiotherapy dose
Chemotherapy | Response assessment | Identified genes: more relevant genes | Outcome |
|
|
Ghadimi et al. 2005 [9] | Tumor tissue biopsy | 30 | No | 50.4 Gy/28 fractions
5FU | Downsizing | 54 genes: SMC1, CLMN, CDC42BPA, and FLNB | Group prediction 83% |
|
|
Watanabe et al. 2006 [10] | Tumor tissue biopsy | 52 | 17 | 50.4 Gy
— | Japanese Classification of Colorectal Carcinoma | 33 genes
(i) Overexpressed: LUM, THBS2, and LGALS1
(ii) Downregulated: CYP40 and GPX2 | Class prediction 82.4%,
sensitivity 50%,
specificity 100%,
PPV 100%,
NPV 76.6% |
|
|
Kim et al. 2007 [11] | Tumor tissue biopsy | 31 | 15 | 50.4 Gy/28 fractions
5FU + LV/capecitabine/
capecitabine + irinotecan | Dworak regression grade | 95 genes: TYMS and RAD23B | Precision 87%,
sensitivity 100%,
specificity 82% |
|
|
Rimkus et al. 2008 [12] | Tumor tissue biopsy | 43 | No | 45 Gy
5FU | Becker regression grade | 42 genes
(i) Overexpressed: CASP1, SLC35E1, CCNK, STAT2, and ETS2
(ii) Downregulated: TDE1, USP42, M-RIP, and FREM1 | Accuracy 81%,
sensitivity 71%,
specificity 86%,
PPV 71%,
NPV 86% |
|
|
Nishioka et al. 2011 [13] | Tumor tissue biopsy | 17 | 3 | 40 Gy/20 fractions
S1 | Japanese Classification of Colorectal Carcinoma | 17 genes: MMP7, MMP14, MMP9, MMP1, MMP16, and RRM1 | |
|
|
Casado et al. 2011 [14] | Tumor tissue biopsy
formalin-fixed paraffin-embedded biopsies | 25
94
| | 50.4 Gy/28 fractions
25 oxaliplatin + raltitrexed
94 different treatment | Dworak regression grade | 24 genes: genetic profile of 13:
(i) 6 overexpressed: ALDH1A1, CDKN1 1, FOS, RELB, STAT3, and TFF3
(ii) 7 downregulated: BAK, MLH1, TYMS, CKB, GPX2, HIG2, and PH-4 | Nonresponders:
accuracy 86%,
sensitivity 87%,
specificity 82% |
|
|
Palma et al. 2013 [8] | Blood sample | 27 | 8 | 50.4 Gy/28 fractions
Capecitabine/capecitabine + oxaliplatin | Mandard regression grade | 8 genes: FALZ | |
|
|
Palma et al. 2014 [7] | Tumor tissue biopsy | 26 | 8 | 50.4 Gy/28 fractions
Capecitabine/capecitabine + oxaliplatin | Mandard regression grade | 257 genes: c-MYC, GNG4, POLA, and RRM1 | Accuracy 85%,
sensitivity 60%,
specificity 100%,
PPV 100%,
NPV 80% |
|
|
Gantt et al. 2014 [15] | Tumor tissue biopsy | 36 | 10 | 50.4 Gy/30 fractions
5FU | American Joint Committee on Cancer | 183 genes up- and downregulated: RAD50 | No response:
sensitivity 33%,
specificity 100% |
|
|
Watanabe et al. 2014 [16] | Tumor tissue biopsy | 46 | 16 | 50.4 Gy/28 fractions
Tegafur-uracil + leucovorin | Japanese Classification of Colorectal Carcinoma | 22 probes (18 genes): signature LRRIQ3, FRMD3, SAMD5, and TMC7 | Accuracy 81.3%,
sensitivity 100%, specificity 62.5%,
PPV 72.7%,
NPV 100% |
|
