Hexosamine-Induced TGF-β Signaling and Osteogenic Differentiation of Dental Pulp Stem Cells Are Dependent on N-Acetylglucosaminyltransferase V

Figure 3

Differential regulation of GlcN/GlcNAc, GalN/GalNAc, and ManN/ManNAc on the mRNA expression of osteogenic genes in dental pulp stem cells (DPSCs). (a) Alkaline phosphatase (ALP) and (b) osteocalcin (OCN). We used cells grown in regular medium as the negative control (NC) and used those in mineralizing medium containing osteogenic supplements (OS) as the positive control. The mRNA levels were assessed by a TaqMan RT-PCR after treatment with various hexosamine derivatives (0.005 mg/mL) for 3, 5, and 7 days. Each of the targeted genes was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Results are expressed as the fold of each experimental group relative to the NC group at day 0 (mean ± SD). .

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