Hexosamine-Induced TGF-<i>β</i> Signaling and Osteogenic Differentiation of Dental Pulp Stem Cells Are Dependent on N-Acetylglucosaminyltransferase V

<div>Effects of SB431542 and SiMgat5 transfection on the expression of TGF-<i>β</i>r type I and Smad2 phosphorylation in DPSCs treated with GlcN/GlcNAc, GalN/GalNAc, and ManN/ManNAc for four hours. We used cells grown in regular medium as the negative control (NC) and those in mineralizing medium containing osteogenic supplements (OS) as the positive control. (a) SB431542 abolished TGF-<i>β</i>r type I expression and Smad2 phosphorylation; (b) transfection with siMgat5 to knockdown GnT-V also abolished TGF-<i>β</i>r type I expression and Smad2 phosphorylation.</div>

BioMed Research International

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Figure 6

Figure 6: Hexosamine-Induced TGF-<i>β</i> Signaling and Osteogenic Differentiation of Dental Pulp Stem Cells Are Dependent on N-Acetylglucosaminyltransferase V 

Figure 6 | Hexosamine-Induced TGF-β Signaling and Osteogenic Differentiation of Dental Pulp Stem Cells Are Dependent on N-Acetylglucosaminyltransferase V 