Biologic Roles of Estrogen Receptor-β and Insulin-Like Growth Factor-2 in Triple-Negative Breast Cancer

Figure 1

ERβ1 expression in archival TNBC specimens. Representative examples are shown of IHC staining of tumor and nonmalignant tissue specimens using anti-ERβ1 antibody (AbDSerotec PPG5/10). (a) TNBC specimen shows nuclear (and cytoplasmic) immunostaining of ERβ1 at low magnification. (b) The same TNBC specimen shows nuclear (and cytoplasmic) immunostaining of ERβ1 at higher magnification. (c) Expression of nuclear ERβ1 is also observed in neighboring nonmalignant mammary tissue from the clinical specimen used in panels (a) and (b). (d) Negative ERβ1 detected in a different clinical specimen as shown as comparison. Antibody binding was detected by using the “Envision+” amplification system followed by chromogen detection with diaminobenzidine (DAKO). Sections were counterstained with Harris hematoxylin followed by dehydration through graded alcohol solutions and mounting. See Table 2 for a summary of findings on all TNBC cases examined.

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