Biologic Roles of Estrogen Receptor-β and Insulin-Like Growth Factor-2 in Triple-Negative Breast Cancer

Figure 3

ERβ is expressed in TNBC cells. Lysates of reported TNBC cells (MDA-MB-231, MDA-MB-435, BT549, HCC38, HCC1143, and HCC1937) and nuclear ERα-positive controls (MCF-7, T47D) were subjected to polyacrylamide gel electrophoresis and immunoblots with anti-ERβ antibody (D7N, Zymed/Invitrogen; confirmed with GeneTex ERβ1 antibody (not shown)) and anti-ERα antibody (1D5, DAKO). T47D cells are also a positive control for ERβ expression. β-actin is used as a loading control. Methods were as before [60, 61]. Blot shown is a representative of at least three independent experiments.