Inhibition of mTOR by Rapamycin Results in Auditory Hair Cell Damage and Decreased Spiral Ganglion Neuron Outgrowth and Neurite Formation In Vitro

Figure 3

Quantitative analysis of surviving OHCs and IHCs treated with rapamycin by cochlear turns. (a) Average surviving OHCs and IHCs in the apical turn with increasing concentrations of rapamycin compared to control. (b) Average surviving OHCs and IHCs in the middle turn with increasing concentrations of rapamycin compared to control. (c) Average surviving OHCs and IHCs in the basal turn with increasing concentrations of rapamycin compared to control. There was a dose dependent statistically significant decrease in IHC and OHC survival in OCs treated with increasing concentrations of rapamycin in all cochlear turns. Asterisks indicate significant difference compared to control (). Data are expressed as the mean number of surviving OHCs and IHCs corresponding to 20 IHCs. Vertical lines represent one standard deviation. for each experimental condition.

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