Repair of Cranial Bone Defects Using rhBMP2 and Submicron Particle of Biphasic Calcium Phosphate Ceramics with Through-Hole

<div>The effect of rhBMP2/d-BCP on osteogenic differentiation in MC3T3-E1 cells. Cells were maintained for 3 days in growth medium with d-BCP (1 mg) or rhBMP2 (0.5 <i>μ</i>g)/d-BCP (1 mg). (a) The cells were subjected to ALP staining. (b) Total RNA was isolated and expression of osteoblast-specific genes was analyzed by RT-PCR. <i>β</i>-actin was used as a loading control. ALP: alkaline phosphatase. OC: osteocalcin. 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BioMed Research International

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Figure 3

Figure 3: Repair of Cranial Bone Defects Using rhBMP2 and Submicron Particle of Biphasic Calcium Phosphate Ceramics with Through-Hole 