Evolution of Action Potential Alternans in Rabbit Heart during Acute Regional Ischemia

Figure 1

Heart instrumentation. (a) Schematic view of the experimental setup. ME1 and ME2—glass microelectrodes impaled to subendo- and subepicardium, respectively. Grey square on the heart indicates the field of view of the camera. EM filter—emission filter. (b) Custom made balloon occluder made of an inflatable silicon tube (shown at higher magnification in the inset) connected to a syringe. (c) Fluorescent image of the coronary circulation after injection of tetramethylrhodamine-isothiocyanate-Dextran (MW40.000) into the aorta. (d) Image of the same area after occlusion. The “x” indicates the artery occlusion site; the white dotted line shows the boundary of the ischemic region.

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