Research Article
Mutations Found in embCAB, embR, and ubiA Genes of Ethambutol-Sensitive and -Resistant Mycobacterium tuberculosis Clinical Isolates from China
Table 2
Characteristics of the mutants in embB, embA, embC, embR, and ubiA within the EMB-resistant isolates.
| | Types | Locus, nucleotide change, and amino acid change |
Number () | High resistance () |
Resistant spectrum
(mean ± SD = 3.49 ± 1.59) | MDR/XDR | | embB | embA | embC | embR | ubiA |
| | 1 | WT | WT | WT | WT | WT | 14 | 0 | 1.8 | 6 none, 8 MDR | | 2 | M306V (ATG-GTG) | WT | WT | WT | WT | 22 | 5 | 1.5 | 9 none, 13 MDR | | 3 | M306L (ATG-CTG) | WT | WT | WT | WT | 8 | 3 | 1.9 | 2 none, 6 MDR | | 4 | M306L (ATG-TTG) | WT | WT | WT | WT | 2 | 1 | 2.2 | 1 none, 1 MDR | | 5 | M306I (ATG-ATA) | WT | WT | WT | WT | 8 | 2 | 3.8 | 3 none
4 MDR, 1 XDR | | 6 | M306I (ATG-ATT) | WT | WT | WT | WT | 1 | 0 | 4 | 1 MDR | | 7 | M306I (ATG-ATT) | WT | WT | WT | E149D (GAA-GAC) | 1 | 1 | 8 | 1 XDR | | 8 | M306I (ATG-ATA)
G406S (GGC-AGC) | WT | WT | WT | WT | 1 | 1 | 5 | MDR | | 9 | M306I (ATG-ATA)
G406D (GGC-GAC) | WT | WT | WT | WT | 1 | 1 | 4 | MDR | | 10 | M306I (ATG-ATA)
G406D (GGC-GAC) | WT | WT | 462 (c-del) | V49L (GTC-CTC)
P254P (CCG-CCT) | 1 | 1 | 4 | MDR | | 11 | M306I (ATG-ATC)
R354S (AGA-AGC) | V122G (GTG-GGG)
V125G (GTG-GGG) | WT | WT | WT | 1 | 1 | 4 | MDR | | 12 | M306V (ATG-GTG) | V343L (GTG-TTG) | WT | WT | WT | 1 | 0 | 6 | MDR | | 13 | M306V (ATG-GTG) | WT | E305D (GAG-GAC) | WT | WT | 1 | 1 | 7 | XDR | | 14 | M306V (ATG-GTG) | WT | WT | G84G (GGT-GGG)
S104N (AGC-AAC) | A38T (GCC-ACC) | 1 | 1 | 5 | MDR | | 15 | M306V (ATG-GTG) | WT | WT | WT | L198L (CTG-CTC) | 1 | 1 | 8 | XDR | | 16 | M306V (ATG-GTG) | WT | WT | WT | I206I (ATC-ATT) | 1 | 0 | 3 | None | | 17 | M306V (ATG-GTG) | L105V (CTG-GTG) | WT | WT | WT | 1 | 0 | 7 | XDR | | 18 | M306V (ATG-GTG) | R380P (CGT-CCT) | WT | WT | WT | 1 | 0 | 3 | MDR | | 19 | M306V (ATG-GTG)
D328H (GAT-CAT) | WT | WT | WT | WT | 1 | 0 | 3 | MDR | | 20 | M306V (ATG-GTG)
D328G (GAT-GGT) | WT | WT | WT | WT | 1 | 1 | 6 | MDR | | 21 | M306V (ATG-GTG)
G406P (GGC-CCG)
A439A (GCA-GCG) | WT | WT | WT | E149D (GAA-GAC) | 1 | 1 | 7 | XDR | | 22 | M306V (ATG-GTG)
G406P (GGC-CCG)
A439A (GCA-GCG) | WT | WT | WT | I206I (ATC-ATT) | 1 | 1 | 6 | MDR | | 23 | M306V (ATG-GTG)
G406A (GGC-GCC) | WT | WT | WT | WT | 1 | 1 | 3 | MDR | | 24 | M306V (ATG-GTG)
Q497R (CAG-CGG) | WT | E305E (GAG-GAA) | WT | WT | 1 | 1 | 4 | MDR | | 25 | M306V (ATG-GTG)
D531D (GAC-GAT) | WT | WT | WT | WT | 4 | 1 | 5.5 | 2 none, 2 MDR | | 26 | D328H (GAT-CAT) | WT | WT | WT | WT | 1 | 0 | 4 | MDR | | 27 | R354S (AGA-AGC) | WT | WT | WT | WT | 3 | 0 | 2 | 1 none, 2 MDR | | 28 | R354S (AGA-AGC)
D531D (GAC-GAT) | WT | WT | WT | WT | 1 | 0 | 3 | None | | 29 | G406P (GGC-CCG) | WT | WT | WT | WT | 1 | 1 | 7 | XDR | | 30 | G406P (GGC-CCG)
A439A (GCA-GCG) | WT | WT | WT | WT | 2 | 0 | 5.5 | MDR | | 31 | G406D (GGC-GAC) | WT | WT | WT | WT | 4 | 2 | 5 | 3 MDR, 1 XDR | | 32 | G406A (GGC-GCC) | WT | WT | WT | WT | 6 | 2 | 5.5 | 5 MDR, 1 XDR | | 33 | G406A (GGC-GCC) | WT | E305E (GAG-GAA) | WT | WT | 1 | 0 | 5 | MDR | | 34 | G406C (GGC-TGC) | WT | WT | WT | WT | 1 | 1 | 4 | MDR | | 35 | A439A (GCA-GCG) | WT | WT | WT | WT | 2 | 0 | 3.5 | 2 none | | 36 | Q497R (CAG-CGG) | WT | WT | WT | WT | 5 | 3 | 5.4 | 2 none, 2 MDR, 1 XDR | | 37 | Q497K (CAG-AAG) | WT | WT | WT | WT | 1 | 1 | 6 | MDR | | 38 | Q497R (CAG-CGG)
T496N (ACC-AAC) | WT | WT | WT | I179T (ATC-ACC) | 1 | 1 | 7 | XDR | | 39 | Q497R (CAG-CGG) | WT | WT | P49A (CCC-GCC) | WT | 1 | 1 | 6 | None | | 40 | WT | WT | WT | P49A (CCC-GCC)
P243S (CCC-TCC) | WT | 1 | 0 | 4 | MDR | | 41 | WT | WT | WT | WT | S244T (AGC-ACC) | 1 | 0 | 5 | MDR |
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WT: wild type; MDR: multidrug resistance; XDR: extensively drug resistance.
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