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BioMed Research International
Volume 2015, Article ID 951871, 14 pages
http://dx.doi.org/10.1155/2015/951871
Research Article

Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus

1Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy
2Institute of Agro-Environment and Forest Biology, National Research Council (CNR), Via Pietro Castellino 111, 80131 Naples, Italy
3Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue 210, 81531-990 Curitiba, PR, Brazil

Received 9 June 2015; Revised 17 September 2015; Accepted 1 October 2015

Academic Editor: Michael E. Kornaros

Copyright © 2015 Rossana Liguori et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

In the supplementary materials are provided: Table S1 indicating the levels decoded of the tested parameters in the Plackett-Burman Screening Design; Table S2 in which are reported the independent factors (Temperature, Time and pH) and their respective coded and decoded levels used for the 33 experimental design of the optimization of the enzymatic hydrolysis of Arundo donax; Table S3 showing the Analysis of Variance (Anova) of Plackett-Burman Screening Design for the enzymatic hydrolysis of Arundo donax; Table S4 showing the Anova of the 33 factorial experimental design for the optimization of enzymatic hydrolysis parameters and Table S5 in which is reported the comparison of observed and predicted values of the 33 factorial experimental design of sugars released (mg/gds) during the enzymatic hydrolysis of Arundo donax. Moreover, they provided: Figure S1 showing the inducer effect of 1% microcrystalline cellulose on cellulase and xylanase activities production by Pleurotus ostreatus in 24-Multiwell; Figure S2 showing the inductive effect of 1% microcrystalline cellulose on cellulase and xylanase activities production by Pleurotus ostreatus in 1‚ÄČL Erlenmeyer flask and Figure S3 in which is reported the correlation between observed and predicted values of the total sugars released by enzymatic hydrolysis of Arundo donax.

  1. Supplementary Material