Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus
Table 1
Plackett-Burman 11/12 screening design: two levels, 6 factors, 3 central points, and 5 dummy factors (D1, D2, D3, D4, and D5).
Run
Temperature (°C)
% biomass (w/v)
pH
Cellulase from PO (U/gds)
Cellulase from Trichoderma reesei ATCC26921 (U/gds)
Time (hours)
Dummy 1
Dummy 2
Dummy 3
Dummy 4
Dummy 5
Total sugars released (mg/gds)
1
45
7
3
10
1.8
24
1
1
1
−1
1
179.15
2
45
3
6
30
1.8
24
−1
1
1
1
−1
509.95
3
25
7
6
10
5.4
24
−1
−1
1
1
1
173.70
4
45
7
3
30
1.8
72
−1
−1
−1
1
1
408.55
5
45
3
6
30
5.4
24
1
−1
−1
−1
1
439.30
6
45
7
6
10
5.4
72
−1
1
−1
−1
−1
312.75
7
25
7
6
30
1.8
72
1
−1
1
−1
−1
112.15
8
25
7
3
30
5.4
24
1
1
−1
1
−1
344.35
9
25
3
3
30
5.4
72
−1
1
1
−1
1
461.70
10
45
3
3
10
5.4
72
1
−1
1
1
−1
598.30
11
25
3
6
10
1.8
72
1
1
−1
1
1
456.35
12
25
3
3
10
1.8
24
−1
−1
−1
−1
−1
271.60
12C
35
5
4.5
20
3.6
48
0
0
0
0
0
387.40
14C
35
5
4.5
20
3.6
48
0
0
0
0
0
419.30
15C
35
5
4.5
20
3.6
48
0
0
0
0
0
355.50
Due to the copresence of two (hemi)cellulolytic activities in the P. ostreatus supernatant, when 10, 20, and 30 U/gds of cellulase are utilized in the different experimental runs, the xylanase concentrations in the enzymatic hydrolytic mixtures are 200, 400, and 600 U/gds, respectively.
