Characteristic Gene Expression Profiles of Human Fibroblasts and Breast Cancer Cells in a Newly Developed Bilateral Coculture System

Figure 1

A concept view of bilateral coculture system and flow of experimental procedures. Breast cancer cells and fibroblasts were cocultured on each side of the bilateral microporous collagen matrix membrane. A suspending two-sided microporous collagen membrane with polystyrene reinforced outer frame was positioned in the culture medium in 50 mm diameter culture vessel. Cells were cultured as shown in the microscopic photo. Analytical flow is as illustrated by employing transcriptomic and bioinformatics analyses.