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BioMed Research International
Volume 2015 (2015), Article ID 962941, 6 pages
http://dx.doi.org/10.1155/2015/962941
Research Article

Amylose-Based Cationic Star Polymers for siRNA Delivery

1Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo, Kyoto 615-8150, Japan
2ERATO Akiyoshi Bio-Nanotransporter Project, JST, Katsura, Nishikyo, Kyoto 615-8150, Japan

Received 11 May 2015; Revised 5 August 2015; Accepted 11 August 2015

Academic Editor: Seung-Wuk Lee

Copyright © 2015 Tomoki Nishimura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A new siRNA delivery system using a cationic glyco-star polymer is described. Spermine-modified 8-arm amylose star polymer (with a degree of polymerization of approximately 60 per arm) was synthesized by chemoenzymatic methods. The cationic star polymer effectively bound to siRNA and formed spherical complexes with an average hydrodynamic diameter of 230 nm. The cationic 8-arm star polymer complexes showed superior cellular uptake characteristics and higher gene silencing effects than a cationic 1-arm polymer. These results suggest that amylose-based star polymers are a promising nanoplatform for glycobiomaterials.