Regulation of Angiogenic Functions by Angiopoietins through Calcium-Dependent Signaling Pathways

Figure 1

is increased by Ang-1 and Ang-2 stimulation. Live imaging in Fura-2-AM-loaded single cells. increases (nM) following stimulation with either Ang-1 or Ang-2 at different concentrations (5 to 400 ng/mL) in Ca²⁺ containing HBSS buffer. Identification of 100 ng/mL Ang-1 and 200 ng/mL Ang-2 as agonist concentrations is most effective in increasing levels (a, b). Data in bar charts represent mean ± s.e.m. from three independent experiments. Statistical analysis of the data was performed using one-way ANOVA test. value < 0.0001, value < 0.05. Lack of contribution from Ca²⁺ influx, tested in absence or presence of extracellular calcium (resp., in KHH or HBSS buffer) (c, d). Changes in levels (nM) are shown as maximum concentration in bar charts (c1, d1) and as representative traces (c2, d2) in cells stimulated with Ang-1 or Ang-2, at the respective maximal concentrations, or with vehicle alone. Agonist-stimulated increases are substantially the same in the presence or absence of extracellular Ca²⁺. Data in bar charts represent mean ± s.e.m. from three independent experiments. Cell number = 78–155. versus vehicle by Student’s -test. values in representative traces are expressed as increase with respect to basal Ca²⁺ concentrations (Δ).

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(b)

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