Regulation of Angiogenic Functions by Angiopoietins through Calcium-Dependent Signaling Pathways

Figure 4

RyR inhibition abates both Ang-1- and Ang-2-mediated intracellular calcium mobilization. Cells were pretreated for 30 min with 30 μM 8Br-cADPR (inactive analogue of cADPR) and then stimulated with Ang-1 or Ang-2 in Ca²⁺-free medium KHH. Histograms show changes in levels in response to each agonist in the presence or absence of the indicated inhibitor (a1, a3). Representative traces showing the effect of 8Br-cADPR on Ang-1-stimulated (a2) and Ang-2-stimulated (a4) cells. Δ on the -axes = nM. Data in bar charts represent mean ± s.e.m. of three independent experiments. Cell number = 96–132. , versus agonist by Student’s -test. values in representative traces are expressed as increase with respect to basal Ca²⁺ concentrations (Δ).